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<t>RT-qPCR</t> detection of CaMKII and Cx43 expression. The relative expression of CaMKII mRNA in the PSD and KN93 groups was significantly higher than that in the control group (both P<0.05), and it was significantly lower in the KN93 group than that in the PSD group (P<0.05). The expression of Cx43 mRNA in the PSD and KN93 groups was significantly lower than that in the control group (both P<0.05), and it was significantly higher in the KN93 group than that in the PSD group (P<0.05). *P<0.05, compared with the respective control group; # P<0.05, compared with the respective PSD group. CaMKII, Ca 2+ /CaM-dependent protein kinase II; Cx43, connexin 43; PSD, post-stroke depression.
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RT-qPCR detection of CaMKII and Cx43 expression. The relative expression of CaMKII mRNA in the PSD and KN93 groups was significantly higher than that in the control group (both P<0.05), and it was significantly lower in the KN93 group than that in the PSD group (P<0.05). The expression of Cx43 mRNA in the PSD and KN93 groups was significantly lower than that in the control group (both P<0.05), and it was significantly higher in the KN93 group than that in the PSD group (P<0.05). *P<0.05, compared with the respective control group; # P<0.05, compared with the respective PSD group. CaMKII, Ca 2+ /CaM-dependent protein kinase II; Cx43, connexin 43; PSD, post-stroke depression.

Journal: Experimental and Therapeutic Medicine

Article Title: Expression and role of CaMKII and Cx43 in a rat model of post-stroke depression

doi: 10.3892/etm.2019.7782

Figure Lengend Snippet: RT-qPCR detection of CaMKII and Cx43 expression. The relative expression of CaMKII mRNA in the PSD and KN93 groups was significantly higher than that in the control group (both P<0.05), and it was significantly lower in the KN93 group than that in the PSD group (P<0.05). The expression of Cx43 mRNA in the PSD and KN93 groups was significantly lower than that in the control group (both P<0.05), and it was significantly higher in the KN93 group than that in the PSD group (P<0.05). *P<0.05, compared with the respective control group; # P<0.05, compared with the respective PSD group. CaMKII, Ca 2+ /CaM-dependent protein kinase II; Cx43, connexin 43; PSD, post-stroke depression.

Article Snippet: TransScript II All-in-One First-Strand cDNA Synthesis SuperMix for PCR and TransScript II SYBR-Green Two-Step RT-qPCR SuperMix kit (AH321-01 and AQ301-01; both from Transgen Biotech Co., Ltd.); RT-qPCR primers [Sangon Biotech (Shanghai) Co., Ltd.]; Trizol kit (10296028; Thermo Fisher Scientific, Inc.); RIPA lysis buffer, BCA kit and ECL chromogenic reagent (P0013C, P0012S, and P0018FS; all from Beyotime Institute of Biotechnology); monoclonal rabbit anti-rat CaMKII, monoclonal rabbit anti-rat Cx43, and monoclonal rabbit anti-rat β-actin antibodies, as well as polyclonal horseradish peroxidase (HRP)-labeled goat anti-rabbit secondary antibody (ab5683, ab79010, ab179467, and ab6728; all from Abcam); KN93 (CSN11255; CSNpharm).

Techniques: Quantitative RT-PCR, Expressing